Ozone Investigation Lesson

 

The Milkweed Alternative

 

Note: Milkweed is only available in the late summer and is not found in every region of the U.S.

Objectives
Students will:

  • observe various milkweed plants and assess for ozone damage
  • compare milkweed data with various other ozone data sources
  • make conclusions about using bioindicators

Materials

locally harvested or observed milkweed leaves
milkweed leaf/Plant Injury Index

Background

Bioindicators are biotic species, (plants, animals, etc.), that display noticeable changes if surrounding environmental conditions change.  Scientists have found that certain organisms can be correlated with certain pollutants.  The changes displayed by the organism are measurable through various methods, therefore can offer quantitative information on ecological resources.

Common Milkweed is considered to be a bioindicator of ozone.  Milkweed shows signs of damage from ozone by showing stiples, or dark polka dots, all over the leaves, losing leaves, turning strange colors, or not growing very big.  Photographs of injured plants can be viewed on the Forest Health Monitoring Program's web site. 

Common milkweed (Asclepias syriaca) usually has a solitary, simple stem (1.5-6 feet tall) though clumps of multiple stems can be found. Leaves are opposite, oval, and 2-10 inches long. The surface of the leaf is hairy beneath and smooth above. The leaf stem is short and thick. Milkweed exudes a thick white sap from any cut or broken surface. Milkweed is common in fields, meadows, and along roadsides from New Brunswick to Saskatchewan, south to Georgia and Tennessee and west to Iowa and Kansas.

Milkweed flowers from June to August. The flower actually consists of many flowers in a large cluster on a stalk. A flower stalk may originate from the tip of a branch or stem or at the junction of a leaf with the stem. The flowers are usually pollinated by insects because the pollen is lumped in waxy masses not conducive to wind fertilization. Very few of the flowers produce large, healthy pods. The distinctive seeds of the milkweed are dispersed by the wind catching the long silky hairs. Studies of seed dispersal indicate seeds typically drift 25 to 100 feet before settling to earth.

There are many other plants that serve as bioindicators for ozone if milkweed is not found in your area.  A brief list includes, Blackberry, Huckelberry, Spreading Dogbane, Black Cherry, Choke Cherry, and Sassafras.  Please consult the Forest Health Monitoring Program’s web site for more bioindicators and information.

Procedure

1. In late August through mid September, select a study area with these characteristics:

a. Plants are at least 50 feet from the road to avoid the direct effects of vehicle exhaust, road salts, and road site mowing. Abandoned fields, remote, unmowed areas in parks or prairies are ideal sites.

b. Have students select a study area that has at least 20 milkweed stems located close together.

**Remember, if the area is on private land, get permission from the landowner to conduct research at that location.**

2.  Have students choose 10 stems at random for the study. Each plant must have a stem with 10 or more leaves on it.  Avoid plants that are diseased or have insect chewing marks on them.


3.  Have students draw a map showing the location of the study area.  The map should show features near the site like roads, trails, signs and buildings that would allow another person to easily find the site.

4. Record findings on the chart.

a. Select first milkweed stem.  Measure and record the height of the stem, base to the top.  Use inches for measurement and record.

b. Count and record the total number of mature leaves on the plant.  Mature leaves measure 2 – 4 inches in length.

c. Count and record the number of leaves showing ozone injury on the plant.

d. Estimate the percentage of leaf area injured.

e. Repeat steps for nine more plants.

Assessment

1. What level of injury did you find?

2. If you found no ozone injury, what might this mean? How can you verify your results?

3. What is a bioindicator? How can using milkweed and other plants as bioindicators help us keep the air clean?

4. What are the advantages or disadvantages to using biomonitoring projects?

 

 

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